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Journal / Bioengineering / Mikrofluidik-Plattform zur Messung der…
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JoVE Journal Bioengineering
Mikrofluidik-Plattform zur Messung der Neutrophilen-Chemotaxis von Rohboden Vollblut
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Mikrofluidik-Plattform zur Messung der Neutrophilen-Chemotaxis von Rohboden Vollblut

Article DOI: 10.3791/51215-v • 10:13 min • June 3rd, 2014
June 3rd, 2014 •
*1,2,3, *1,2,3, 1, 1, 1,4, 1,2,3
1The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences
* These authors contributed equally

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Dieses Protokoll Details einen Test entwickelt, um menschliche Neutrophilen-Chemotaxis von einem Tropfen Vollblut mit robusten Reproduzierbarkeit messen. Dieser Ansatz umgeht die Notwendigkeit der Trennung von Neutrophilen und nur wenige Minuten von Assay Vorbereitungszeit erfordert. Die Mikrofluidik-Chip ermöglicht die wiederholte Messung der Neutrophilen-Chemotaxis über die Zeit bei Säuglingen oder Kleinsäuger, wo Probenvolumen ist begrenzt.

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Bioengineering Chemotaxis Neutrophilen Vollbluttest Mikrofluidik-Vorrichtung chemoattractant Migration Entzündung
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