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Journal / Neuroscience / Brain Slice Biotinylatie: An
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Brain Slice Biotinylatie
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Brain Slice Biotinylatie: An

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Neuronale membraan mensenhandel stelt dynamisch plasmamembraaneiwit beschikbaarheid en significant invloed neurotransmissie. Tot op heden, is het een uitdaging om neuronale endocytische handel in volwassen neuronen te meten. Hier beschrijven we een zeer effectieve, kwantitatieve methode om snelle veranderingen in de oppervlakte-eiwit expressie ex vivo bij acute plakjes hersenen te meten.

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Neurowetenschappen mensenhandel endocytose internalisatie biotinylatie hersenen neuronen transporter proteïne kinase C
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