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Journal / Neuroscience / Slice Cervello biotinilazione: An
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Slice Cervello biotinilazione
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Slice Cervello biotinilazione: An

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Traffico di membrana neuronale controlla dinamicamente la membrana plasmatica disponibilità di proteine ​​e gli impatti in modo significativo la neurotrasmissione. Ad oggi, è stato impegnativo per misurare neuronale traffico endocitico in neuroni adulti. Qui, descriviamo un metodo quantitativo altamente efficace per misurare rapidi cambiamenti nell'espressione della proteina superficie ex vivo in fettine cerebrali acute.

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Neuroscienze Numero 86 Traffico endocitosi interiorizzazione biotinilazione il cervello i neuroni trasportatore proteina chinasi C
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