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Journal / Biology / Generering af Myospheres Fra hESCs ved…
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Generering af Myospheres Fra hESCs ved Epigenetisk Omprogrammering
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Generering af Myospheres Fra hESCs ved Epigenetisk Omprogrammering

Article DOI: 10.3791/51243-v • 09:32 min • June 21st, 2014
June 21st, 2014 •
1, 1,2
1Muscle Development and Regeneration Program, Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

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Her beskriver vi en protokol baseret på epigenetiske omprogrammering af humane embryonale stamceller (hESCs) retning af at skabe en homogen population af skeletmuskulaturen stamfædre, at der under tolerante dyrkningsbetingelser danner tredimensionelle klynger af kontraktile myofibre (myospheres), som rekapitulere biologiske funktioner i menneskets skelet muskler.

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Bioteknik væv celler Embryonale Konstruktioner bevægeapparatet muskel-og knoglesygdomme embryonale epinegetics Skeletal Myogenesis Myosphere kromatin Lentivirus infektion
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