Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Helical Organization of Blood Coagulation Factor…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
The Muscle Cuff Regenerative Peripheral Nerve Interface for the Amplification of Intact Peripheral Nerve Signals
Journal (Bioengineering)
The Muscle Cuff Regenerative Peripheral Nerve Interface for the Amplification of Intact Peripheral Nerve Signals
Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management
Journal (Bioengineering)
Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management
 

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

Chapters

Summary
Automatic Translation

We present a combination of Cryo-electron microscopy, lipid nanotechnology, and structure analysis applied to resolve the membrane-bound structure of two highly homologous FVIII forms: human and porcine. The methodology developed in our laboratory to helically organize the two functional recombinant FVIII forms on negatively charged lipid nanotubes (LNT) is described.

Tags

Cryo-electron Microscopy Lipid Nanotubes Coagulation Factor VIII FVIII Hemophilia Type A Serine Protease Blood Clotting Membrane-bound Structure Recombinant FVIII Human Factor IXa Porcine FVIII Structure Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter