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JoVE Journal Bioengineering
Helical Organisering av koagulasjonsfaktor VIII på Lipid Nanorør
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Helical Organisering av koagulasjonsfaktor VIII på Lipid Nanorør

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

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Vi beskriver her en kombinasjon av Cryo-elektronmikroskopi, lipid nanoteknologi, og struktur-analyse anvendt for å løse membranbundet struktur av to sterkt homologe FVIII former: humane og porcine. Metodikken er utviklet i vårt laboratorium for å helically organisere de to funksjonelle rekombinante FVIII skjemaer på negativt ladet lipid nanorør (LNT) er beskrevet.

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Bioteknologi Cryo-elektron mikroskopi Lipid nanorør Helical montering Membran-bundet organisasjon koagulasjonsfaktor VIII
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