JoVE Journal
Immunology and Infection
Immunology and Infection
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Summary
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树突状细胞(DC)分泌IL-1β响应于TLR8识别合成嘌呤,R848,接着NLRP3炎性体激活与尼日利亚菌素,因此,IL-1β可以用来测量NLRP3炎性活性。细胞内细胞因子染色法,免疫印迹和ELISA法用于准确地测量通过IL-1β表达NLRP3炎性引发和活化。