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JoVE Journal
Biology

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测定蛋白酶体降解的植物无细胞系统
 
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测定蛋白酶体降解的植物无细胞系统

Article DOI: 10.3791/51293-v 07:43 min March 26th, 2014
March 26th, 2014

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

有针对性的蛋白质降解是一个重大的监管机制,细胞的功能。它发生通过一个保守的泛素-蛋白酶体通路,其高度多聚泛素链的靶蛋白,然后作为分子的“标签”为26S蛋白酶体。在这里,我们描述了用于蛋白质的蛋白酶体降解的简单和可靠的无细胞测定法。

Tags

生物化学,第85,泛素/蛋白酶体系统,26S蛋白酶,蛋白质降解,蛋白酶体抑制剂,免疫印迹,植物遗传转化
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