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JoVE Journal Bioengineering
Ekspression, isolation og oprensning af opløselige og uopløselige Biotinylerede Proteiner til nervevæv Regeneration
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Ekspression, isolation og oprensning af opløselige og uopløselige Biotinylerede Proteiner til nervevæv Regeneration

Article DOI: 10.3791/51295-v • 12:03 min • January 22nd, 2014
January 22nd, 2014 •
1, 1, 1, 1
1Department of Chemical and Biomolecular Engineering, University of Akron

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Udvikling biotinylatable fusionsproteiner har mange potentielle anvendelsesmuligheder på forskellige forskningsområder. Rekombinant protein engineering er en ligetil procedure, der er omkostningseffektiv, der giver høje udbytter af specialdesignede proteiner.

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Bioteknik protein engineering produktion af rekombinante proteiner AviTag BirA biotinylering pET vektor-system, Ni-NTA gelpermeationskromatografi
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