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Journal / Bioengineering / Elektrisk celle-substrat Impedans Sensing til…
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Bioengineering

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JoVE Journal Bioengineering
Elektrisk celle-substrat Impedans Sensing til kvantificering af endotelproliferation, barrierefunktion, og motilitet
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Elektrisk celle-substrat Impedans Sensing til kvantificering af endotelproliferation, barrierefunktion, og motilitet

Article DOI: 10.3791/51300-v • 12:30 min • March 28th, 2014
March 28th, 2014 •
1, 1, 2
1Department of Pulmonary Diseases, Institute for Cardiovascular Research, VU University Medical Center, 2Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center

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Denne protokol anmeldelser Electric Cell-substrat Impedans Sensing, en metode til at registrere og analysere impedans spektrum af klæbende celler til kvantificering af cellevedhæftning, proliferation, bevægelighed og cellulære reaktioner på farmakologiske og giftige stimuli. Påvisning af endotelpermeabilitet og vurdering af celle-celle og celle-substrat kontakter fremhæves.

Tags

Bioteknik ECIS impedansspektroskopi Resistance TEER endotel Barrier celleadhæsioner Focal sammenvoksninger formering migrering motilitet sårheling
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