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Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Article DOI: 10.3791/51305-v • 08:27 min • April 16th, 2014
April 16th, 2014 •
1,2, 1,2, 3, 1,2,4, 1,2, 1,2
1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota

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Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.

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Caspase Multiplexing Assay Apoptosis Hypothalamic Cell Model Multiplex Assay Palmitic Acid Challenge Fluorescent Assay Luminescent Assay Viable Cell Counts Caspase-3/7 Activity 96-well Microtiter Plate Format Resazurin-based Assay DEVD Substrate Change In Caspase Activity Apoptotic Stimulus Saturated Fatty Acid Challenge Oxidative Stress Neuronal Function
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