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JoVE Journal Bioengineering
Brug af Label-fri Optiske Biosensors Detect Modulation af kalium kanaler af G-protein koblede receptorer
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Brug af Label-fri Optiske Biosensors Detect Modulation af kalium kanaler af G-protein koblede receptorer

Article DOI: 10.3791/51307-v • 10:59 min • February 10th, 2014
February 10th, 2014 •
1, 3, 1,2
1Department of Pharmacology, Yale School of Medicine, 2Department of Cellular and Molecular Physiology, Yale School of Medicine, 3X-BODY Biosciences

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Optisk biosensor teknikker kan detektere ændringer i massen nær plasmamembranen i levende celler og tillader en at følge cellulære reaktioner i både individuelle celler og populationer af celler. Denne protokol vil beskrive detektion af modulation af kaliumkanaler af G-protein-koblede receptorer i intakte celler ved hjælp af denne fremgangsmåde.

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Bioteknik Ionkanaler kalium kanal slap G-protein-koblede receptorer (GPCR) etiket-fri screening high-throughput screening (HTS) kanal-protein interaktioner optiske biosensorer
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