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Analysis of RNA Processing Reactions Using Cell Free Systems
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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Article DOI: 10.3791/51309-v • 09:16 min • May 3rd, 2014
May 3rd, 2014 •
1, 1, 2, 1
1Department of Infectious Diseases, The Scripps Research Institute, 2Department of Chemistry, City College of New York

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Summary
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RNA polymerase II synthesizes a precursor RNA that extends beyond the 3' end of the mature mRNA. The end of the mature RNA is generated cotranscriptionally, at a site dictated by RNA sequences, via the endonuclease activity of the cleavage complex. Here, we detail the method to study cleavage reactions in vitro.

Tags

RNA Processing Cell Free Systems 3' End Cleavage Precursor MRNA RNA Polymerase II Endonuclease Activity PolyA Site Cleavage Complex RNA Sequences Polyadenylation PolyA Polymerase Radiolabeled RNA Substrates Gel Electrophoresis Autoradiography HIV-1 MRNAs
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