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Journal / Neuroscience / Neonatal PIAL Surface Electroporation
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JoVE Journal Neuroscience
Neonatal PIAL Surface Electroporation
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Neonatal PIAL Surface Electroporation

Article DOI: 10.3791/51319-v • 06:22 min • May 7th, 2014
May 7th, 2014 •
1, 2, *1, *2
1Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Regenerative Medicine Institute, Cedars-Sinai Medical Center
* These authors contributed equally

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Pial overflaten er en unik stamfar sone i CNS som får stadig større oppmerksomhet. Heri, we detalj en fremgangsmåte for hurtig genetisk manipulering av denne progenitor sonen ved hjelp av en modifisert metode electroporation. Denne prosedyren kan brukes til cellulære og molekylære undersøkelser av celle linjene og signalveier er involvert i celledifferensiering og å belyse skjebnen og egenskaper av datterceller.

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Neuroscience Developmental Biology neonatal gnager skjebne kartlegging avstamning tracing genetisk manipulasjon plasmid DNA piggyBac tol2 transposon TCHD elektroporering
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