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Una rapida e specifica Microplate Assay per la determinazione di ascorbato intra-ed extracellulare in cellule in coltura
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Una rapida e specifica Microplate Assay per la determinazione di ascorbato intra-ed extracellulare in cellule in coltura

Article DOI: 10.3791/51322-v • 11:56 min • April 11th, 2014
April 11th, 2014 •
1, 2
1Molecular Pharmacology and Pathology Program, Department of Pathology & Bosch Institute, University of Sydney, 2Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University

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L'ascorbato interpreta numerosi ruoli importanti nel metabolismo cellulare, molti dei quali sono venuti alla luce solo negli ultimi anni. Qui si descrive un medium-throughput specifico e poco costoso saggio micropiastra per la determinazione di entrambi ascorbato intra ed extracellulare in coltura cellulare.

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Biochimica Vitamina C ascorbato gonfiore delle cellule glutammato analisi micropiastre astrociti
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