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Interazioni proteina-proteina visualizzati tramite bimolecular complementazione fluorescenza in protoplasti di tabacco e foglie
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Interazioni proteina-proteina visualizzati tramite bimolecular complementazione fluorescenza in protoplasti di tabacco e foglie

Article DOI: 10.3791/51327-v • 11:10 min • March 9th, 2014
March 9th, 2014 •
1, 1
1Department Biologie I, Botanik, Ludwig-Maximilians-Universität, München

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Formazione di complessi proteici in vivo può essere visualizzato da bimolecular fluorescenza complementazione. Partner di interazione sono fusi a parti complementari tag fluorescenti e transitoriamente espressi in foglie di tabacco, risultando in un segnale fluorescente ricostituito upon vicinanza delle due proteine.

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Biologia Vegetale Numero 85 Tetratricopeptide dominio ripetizione chaperone cloroplasti reticolo endoplasmatico HSP90 complesso Toc Sec translocon BiFC
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