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JoVE Journal Neuroscience
Konsensus Brain-protein, Extraction protokollen for Studiet af humane og murine Brain Proteome Brug både 2D-DIGE og Mini 2DE Immunoblotting
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Konsensus Brain-protein, Extraction protokollen for Studiet af humane og murine Brain Proteome Brug både 2D-DIGE og Mini 2DE Immunoblotting

Article DOI: 10.3791/51339-v • 10:51 min • April 10th, 2014
April 10th, 2014 •
1, 1,2, 1, 1, 1, 1, 1, 3, 4, 2, 3, 1, 1, 1, 1, 1
1Team Alzheimer & Tauopathies, Jean-Pierre Aubert Research Centre, Inserm UMR 837, 2EA 4308-Department of Reproductive Biology-Spermiology-CECOS, CHRU-Lille, 3EA2686-Laboratorie d'Immunologie, Faculté de Médecine - Pôle Recherche, 4Department of Neurology, CHRU-Lille

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En almindelig proteinekstraktion protokol hjælp urea / thiourinstof / SDS buffer for mennesker og mus hjernevæv tillader Identifikation af proteiner ved 2D-DIGE og deres efterfølgende karakterisering af mini 2DE immunoblotting. Denne metode gør det muligt at opnå mere reproducerbare og pålidelige resultater fra humane biopsier og eksperimentelle modeller.

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Neuroscience proteomik neurodegeneration 2DE menneskelige og mus hjernevæv fluorescens immunblotting. 2D-DIGE (todimensional fluorescens forskel gelelektroforese) mini-2DE (mini 2DE immunoblotting) IPG (Immobiliserede pH-gradienter) IEF (isoelektrofokusering) AD (Alzheimers sygdom )
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