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JoVE Journal Bioengineering
La lignina down-regulation dei
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La lignina down-regulation dei

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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Un doppio filamento di RNA interference (dsRNAi) tecnica viene impiegata per down-regolare il coenzima mais cinnamoil A reduttasi (ZmCCR1) gene a basso contenuto di lignina pianta. La lignina down-regulation dalla parete cellulare viene visualizzato mediante analisi al microscopio e quantificato con il metodo Klason. Cambiamenti di composizione in emicellulosa e cellulosa cristallina vengono analizzati.

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Bioingegneria , Cinnamoil-CoA reduttasi (CCR) dsRNAi misura lignina Klason cella di analisi parete di carboidrati gas-cromatografia (GC)
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