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Immunology and Infection

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JoVE Journal Immunology and Infection
Identification de nouveaux gènes associés à la production d'alginate dans
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Identification de nouveaux gènes associés à la production d'alginate dans

Article DOI: 10.3791/51346-v • 08:57 min • March 10th, 2014
March 10th, 2014 •
1, 1, 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

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Ici, nous décrivons un protocole en utilisant la mini-himar1 marin mutagenèse de transposon médiation pour générer une bibliothèque de mutants d'insertion à haute densité à l'écran, isoler et d'identifier de nouveaux régulateurs d'alginate dans le prototype Pseudomonas aeruginosa souche PAO1.

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Immunologie Numéro 85, L'alginate mucoidy mutagenèse mini- PFA
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