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Journal / Immunology and Infection / Identifikation af nye gener forbundet med Alginat …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation af nye gener forbundet med Alginat Produktion i
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Selective Harvesting of Marginating-pulmonary Leukocytes
Journal (Immunology and Infection)
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Identifikation af nye gener forbundet med Alginat Produktion i

Article DOI: 10.3791/51346-v • 08:57 min • March 10th, 2014
March 10th, 2014 •
1, 1, 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

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Her beskriver vi en protokol ved hjælp af mini-himar1 mariner transposon-medieret mutagenese for at generere et high-density indsættelse mutantbibliotek til skærm, isolere og identificere nye alginat lovgivere i det prototypiske Pseudomonas aeruginosa stamme PAO1.

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Immunologi , Alginat mucoidy mutagenese mini- pFAC
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