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Immunology and Infection

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JoVE Journal Immunology and Infection
Identificazione di geni novelli associati con alginato di produzione in
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Identificazione di geni novelli associati con alginato di produzione in

Article DOI: 10.3791/51346-v • 08:57 min • March 10th, 2014
March 10th, 2014 •
1, 1, 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

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Qui si descrive un protocollo con il mini-himar1 mariner trasposone-mediata mutagenesi per generare una libreria mutante inserimento ad alta densità per schermo, isolare ed identificare i regolatori alginato romanzo nel prototipo Pseudomonas aeruginosa ceppo PAO1.

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Immunologia Numero 85, Alginato mucoidy mutagenesi mini- pFAC
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