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Glycopeptides Capture pour protéomique de surface cellulaire
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Glycopeptides Capture pour protéomique de surface cellulaire

Article DOI: 10.3791/51349-v • 10:11 min • May 9th, 2014
May 9th, 2014 •
1, 1
1Department of Chemistry, Simon Fraser University

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les protéines de surface cellulaire sont biologiquement importantes et largement glycosylée. Nous présentons ici une approche glycopeptides capture pour solubiliser, enrichir et deglycosylate ces protéines pour les analyses protéomiques faciles à base LC-MS.

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Molecular Biology numéro 87 la protéine de membrane une glycoprotéine N-liée à la modification post-traductionnelle la spectrométrie de masse HPLC la chimie hydrazide N-glycoprotéomique glycopeptide capture
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