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En strategi for Sensitive, Large Scale Kvantitative Metabolomics
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En strategi for Sensitive, Large Scale Kvantitative Metabolomics

Article DOI: 10.3791/51358-v • 14:18 min • May 27th, 2014
May 27th, 2014 •
1, 1, 1,2, 1,2, 1,2
1Division of Nutritional Sciences, Cornell University, 2Field of Biochemistry, and Molecular Cell Biology, Cornell University

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Metabolitt profilering har vært en verdifull ressurs i studiet av stoffskiftet i helse og sykdom. Ved hjelp av normal-faset væskekromatografi koblet til høy-oppløsningsmassespektroskopi med polaritet svitsjing og en hurtig arbeidssyklus beskrives en protokoll for å analysere den polare metabolske Sammensetningen av det biologiske materiale med høy følsomhet, nøyaktighet og oppløsning.

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Kjemi høyoppløselig massespektrometri metabolomics positiv / negativ veksling lav masse kalibrering Orbitrap
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