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Journal / Neuroscience / Ved hjælp af en α-Bungarotoxin-bindingssted Tag…
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Ved hjælp af en α-Bungarotoxin-bindingssted Tag til Study GABAA receptor membranlokalisering og handel
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Ved hjælp af en α-Bungarotoxin-bindingssted Tag til Study GABAA receptor membranlokalisering og handel

Article DOI: 10.3791/51365-v • 11:57 min • March 28th, 2014
March 28th, 2014 •
1, 1, 1
1Pharmacology & Chemical Biology Department, University of Pittsburgh School of Medicine

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Her har vi demonstrere brugen af ​​fluorescerende Alexa farvestof koblet til α-bungarotoxin at måle GABAA receptor overflade lokalisering og endocytose i hippocampus neuroner. Gennem brug af konstruktionerne, der bærer en kort ekstracellulære tag, der binder α-bungarotoxin, kan opnås analyse af plasmamembranprotein endocytiske handel.

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Neuroscience α-bungarotoxin bindingssted endocytose immunfarvning gnaver hippocampus neuroner receptor menneskehandel plasmamembranen
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