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JoVE Journal
Neuroscience

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Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
 

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Article DOI: 10.3791/51382-v 07:49 min July 7th, 2014
July 7th, 2014

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Summary

To obtain high-resolution images of fluorescently labeled cells within large tissues, ideally, the biological samples should be imaged without sectioning. 3DISCO is a straightforward tissue clearing procedure based on sequential incubation with organic solvents. Upon clearing, the organs become transparent allowing an end-to-end laser scan of the specimen.

Tags

Tissue Clearing 3DISCO 3D Imaging Transparent Organs Fluorescent Imaging Refractory Indices Laser-scanning Microscopes High-resolution 3D Image Tissue Clearing Protocols CLARITY Sca/e ClearT2 SeeDB Microscopy Techniques Applications Limitations Difficulties
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