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Bio-layer interferometrie voor het meten van kinetiek van eiwit-eiwit interacties en Allosteric Ligand Effects
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Bio-layer interferometrie voor het meten van kinetiek van eiwit-eiwit interacties en Allosteric Ligand Effects

Article DOI: 10.3791/51383-v • 13:57 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

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De protocollen beschrijven hier kinetische analyses van eiwit-eiwit interacties met Bio-layer interferometrie. F type ATP synthase, dat betrokken is bij de cellulaire energiemetabolisme, kan worden geremd door de ε subeenheid in bacteriën. We hebben Bio-layer interferometrie aangepast aan interacties van de katalytische complex studeren met remmende C-terminale domein ε's.

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Chemie ATP synthase Bio-Layer interferometrie ligand-geïnduceerde conformationele verandering Biomoleculaire Interaction Analysis Allosteric regelgeving enzyminhibitie
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