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Bio-Schicht-Interferometrie zur Messung der Kinetik der Protein-Protein-Interaktionen und allosterische Ligandeneffekte
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Bio-Schicht-Interferometrie zur Messung der Kinetik der Protein-Protein-Interaktionen und allosterische Ligandeneffekte

Article DOI: 10.3791/51383-v • 13:57 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

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Die Protokolle beschreiben kinetische Untersuchungen von Protein-Protein-Wechselwirkungen mit Bio-Schicht-Interferometrie. F-ATP-Synthase, die in der zellulären Energiestoffwechsel beteiligt ist, kann durch die ε-Untereinheit in Bakterien gehemmt werden. Wir haben Bio-Schicht Interferometrie geeignet ist, Wechselwirkungen des katalytischen Komplexes mit inhibitorischer C-terminalen Domäne ε zu studieren.

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Chemie Heft 84 ATP-Synthase Bio-Layer-Interferometrie Ligand-induzierten Konformationsänderung Biomolekulare Interaktionsanalyse allosterische Regulierung Enzymhemmung
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