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BEST: Barcode Enabled Sequencing van Tetrads

Article DOI: 10.3791/51401-v • 12:59 min • May 1st, 2014
May 1st, 2014 •
1, 1, 1, 1
1Pacific Northwest Diabetes Research Institute

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Barcode Enabled Sequencing van Tetrads (BEST) vervangt de handmatige processen te isoleren, verstoren en spacing tetraden. BESTE isolaten tetrads door fluorescentie-geactiveerde celsortering op agarplaten, scheidt de sporen door roeren met glaskralen, en bepaalt welke willekeurig bekleed kolonies afgeleid van dezelfde oorspronkelijke viertal behulp van moleculaire barcodes.

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Genetica Gist Viertal Genetica DNA-sequencing
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