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JoVE Journal
Chemistry

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定量蛋白质组学使用还原二甲基化的稳定同位素标记
 
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定量蛋白质组学使用还原二甲基化的稳定同位素标记

Article DOI: 10.3791/51416-v 11:53 min July 1st, 2014
July 1st, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

肽的还原性二甲基化(REDI标签)稳定同位素标记是一种快速,廉价的战略精确质谱为基础的定量蛋白质组学。这里我们展示了一个可靠的方法用于制备使用可以应用于几乎任何类型的样品中的Redi途径的蛋白质混合物的分析。

Tags

化学,第89期,定量蛋白质组学,质谱,稳定同位素,还原二甲基化,肽标记,LC-MS/MS
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