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Journal / Chemistry / Kvantitative Proteomics anvendelse af reduktiv…
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JoVE Journal Chemistry
Kvantitative Proteomics anvendelse af reduktiv Dimethylation for mærkning med stabile isotoper
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Journal (Chemistry)
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Journal (Chemistry)
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
 
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Kvantitative Proteomics anvendelse af reduktiv Dimethylation for mærkning med stabile isotoper

Article DOI: 10.3791/51416-v • 11:53 min • July 1st, 2014
July 1st, 2014 •
1,2,3, 4
1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center

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Stabil isotop mærkning af peptider ved reduktiv dimethylation (redi mærkning) er en hurtig, billig strategi for nøjagtige massespektrometri-baserede kvantitative proteomics. Her demonstrerer vi en robust metode til forberedelse og analyse af proteinblandinger hjælp Redi tilgang, der kan anvendes til næsten enhver prøvetype.

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Kemi kvantitative proteomics massespektrometri stabil isotop reduktiv dimethylation peptid mærkning LC-MS/MS
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