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El aislamiento de los pequeños RNAs no codificantes de suero humano
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El aislamiento de los pequeños RNAs no codificantes de suero humano

Article DOI: 10.3791/51443-v • 06:44 min • June 19th, 2014
June 19th, 2014 •
1, 1, 2,3
1School of Medical and Molecular Biosciences, Faculty of Science, University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital

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Este protocolo describe un método para la extracción de ARN pequeños a partir de suero humano. Hemos utilizado este método para aislar los microARNs de suero de cáncer para su uso en arrays de ADN y PCR cuantitativa singleplex. El protocolo utiliza fenol y tiocianato de guanidina reactivos con modificaciones para producir ARN de alta calidad.

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Bioingeniería Número 88 aislamiento en pequeños no codificante ARN microRNAs suero humano qPCR tiocianato de guanidina Fase Geles Lock arrays
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