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Polysomprofiler Fraktionering og analyse af pattedyr Translatomes på en genom-plan
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Polysomprofiler Fraktionering og analyse af pattedyr Translatomes på en genom-plan

Article DOI: 10.3791/51455-v • 10:56 min • May 17th, 2014
May 17th, 2014 •
1, 2, 3, 3, 1, 2, 1
1Lady Davis Institute and Department of Oncology, McGill University, 2Department of Oncology-Pathology, Karolinska Institutet, 3Goodman Cancer Centre and Department of Biochemistry, McGill University

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Ribosomer spiller en central rolle i proteinsyntesen. Polyribosome (polysom) fraktionering af saccharosedensitetsgradientcentrifugering giver mulighed for direkte bestemmelse af oversættelse effektiviteten af ​​individuelle mRNAer på en genom-plan. Desuden kan denne metode anvendes til biokemisk analyse af ribosom-og polysom-associerede faktorer såsom chaperoner og signalstoffer.

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Biokemi Celler Eukaryota Ernærings-og stofskiftesygdomme neoplasmer Metabolic Phenomena Cell Physiological Phenomena mRNA oversættelse ribosomer proteinsyntese genom-dækkende analyse translatome mTOR eIF4E 4E-BP1
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