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Hot Biologische Katalyse: Isotherme Titrationskalorimetrie zu Enzymreaktionen Charakterisieren

Article DOI: 10.3791/51487-v • 13:00 min • April 4th, 2014
April 4th, 2014 •
1, 1, 1
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna

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Isotherme Titrationskalorimetrie Maßnahmen Wärmestrom freigegeben oder in chemischen Reaktionen absorbiert. Dieses Verfahren kann verwendet werden, um Enzym-Katalyse zu quantifizieren. In diesem Papier, das Protokoll für die Instrumental Setup-Experiment läuft, und Datenanalyse wird allgemein beschrieben, und der Charakterisierung der enzymatischen Hydrolyse von Harnstoff Bohne Urease aufgebracht.

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Chemie Heft 86 Isotherme Titrationskalorimetrie enzymatische Katalyse Kinetik Thermodynamik Enthalpie Michaelis-Konstante katalytische Geschwindigkeitskonstante Urease
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