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Aip1p Dynamics ändras genom den R256H Mutation i aktin
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Journal (Biology)
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Aip1p Dynamics ändras genom den R256H Mutation i aktin

Article DOI: 10.3791/51551-v • 08:57 min • July 30th, 2014
July 30th, 2014 •
1, 2, 2, 1,2
1Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, 2Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, University of Iowa

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Sjukdomsframkallande mutationer i aktin kan förändra cytoskeletal funktion. Cytoskelettala dynamik kvantifieras genom avbildning av fluorescerande taggade proteiner med hjälp av total inre fluorescensmikroskopi. Som ett exempel har den cytoskelettprotein, Aip1p, förändrad lokalisering och rörelse i celler som uttrycker den mutanta aktin isoformen, R256H.

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Developmental Biology grönt fluorescerande protein aktin Aip1p total inre fluorescensmikroskopi jäst kloning
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