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JoVE Journal
Biology

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提取毒液和毒腺Microdissections从蜘蛛的蛋白质组学和转录组分析
 
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提取毒液和毒腺Microdissections从蜘蛛的蛋白质组学和转录组分析

Article DOI: 10.3791/51618-v 10:25 min November 3rd, 2014
November 3rd, 2014

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Summary

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Click here for the English version.

本文提供了一个协议,使用电刺激蜘蛛毒液为了提取1)进行蛋白质鉴定,2)刺激毒腺基因的表达; 3)执行毒液的功能研究。这之后的毒腺microdissections用于基因表达研究的描述。

Tags

遗传学,第93,蜘蛛,毒素,蛋白组学,转录组,电刺激,
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