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JoVE Journal Biology
Isolamento de Neonatal extra-hepáticas cholangiocytes
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Isolamento de Neonatal extra-hepáticas cholangiocytes

Article DOI: 10.3791/51621-v • 07:54 min • June 5th, 2014
June 5th, 2014 •
1, 2
1Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, 2Department of Medicine, The Perelman School of Medicine at the University of Pennsylvania

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Uma técnica para isolar cholangiocytes das vias biliares extra-hepáticos de ratos neonatais é descrito. As condutas são meticulosamente dissecados, e, em seguida, as células são isoladas por crescimento em géis de colagénio espessas. Este método fornece uma ferramenta útil para estudar o desenvolvimento do ducto biliar extra-hepática e patologia.

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Medicina Edição 88 Vias Biliares biliares extra-hepáticas Dutos Duto Biliar Comum Doenças dos Ductos Biliares cultura celular do ducto biliar atresia biliar fígado vesícula biliar fibrose
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