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Immunology and Infection

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JoVE Journal Immunology and Infection
L'uso di fluorescenti target matrici per la valutazione delle cellule T risposte
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L'uso di fluorescenti target matrici per la valutazione delle cellule T risposte

Article DOI: 10.3791/51627-v • 12:09 min • June 19th, 2014
June 19th, 2014 •
1, 1, 1, 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University

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La capacità di monitorare le risposte delle cellule T in dettaglio in vivo è importante per lo sviluppo della nostra comprensione della risposta immunitaria. Qui si descrive l'uso di array di destinazione fluorescenti (ALS) in un test in vivo di cellule T in che valuta> 250 parametri contemporaneamente mediante citometria a flusso.

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Immunologia Numero 88 tecniche investigative la risposta delle cellule T Citometria a Flusso Multiparameter saggio CTL Carbossifluoresceina estere succinimidil (CFSE) CellTrace Violet (CTV) Cell Proliferation Dye eFluor 670 (CPD)
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