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JoVE Journal Immunology and Infection
O Uso de fluorescentes alvo Matrizes para a Avaliação de respostas de células T
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O Uso de fluorescentes alvo Matrizes para a Avaliação de respostas de células T

Article DOI: 10.3791/51627-v • 12:09 min • June 19th, 2014
June 19th, 2014 •
1, 1, 1, 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University

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A capacidade de monitorar as respostas das células T em detalhe, in vivo é importante para o desenvolvimento da nossa compreensão da resposta imune. Aqui nós descrevemos o uso de matrizes alvo fluorescentes (ACL) em um ensaio in vivo de células T em que avalia> 250 parâmetros simultaneamente por citometria de fluxo.

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Imunologia Edição 88 Técnicas de Pesquisa a resposta das células T Citometria de Fluxo Multi ensaio CTL Éster succinimidyl carboxifluoresceína (CFSE) CellTrace Violet (CTV) Cell Proliferação Dye eFluor 670 (CPD)
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