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Kombineret DNA-RNA Fluorescent
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Kombineret DNA-RNA Fluorescent

Article DOI: 10.3791/51628-v • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Fluorescerende in situ-hybridisering (FISH) muliggør påvisning af nukleinsyrer i deres native miljø i cellerne. Vi her beskrive en protokol for den kombinerede, samtidig påvisning af RNA og DNA ved hjælp af fisk, som kan bruges til at studere X-kromosom inaktivering i muse embryonale stamceller.

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Biokemi Fluorescent kombineret DNA-RNA FISH ES celle cytogenetik enkelt celle analyse X-kromosom inaktivering (XCI) DNA-probe,
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