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Journal / Biology / Én-kanals Cell-attached Patch-clamp optagelse
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Én-kanals Cell-attached Patch-clamp optagelse

Article DOI: 10.3791/51629-v • 13:07 min • June 9th, 2014
June 9th, 2014 •
1, 2, 1, 3, 4
1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY

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Beskrevet her er en procedure for at opnå lange strækninger af aktuelle optagelse fra en ion-kanal med celle-attached patch-clamp teknik. Denne metode giver mulighed for at observere, i realtid, mønsteret for open-close kanal konformationer, der ligger til grund for biologisk signal. Disse data informere om kanal ejendomme i uforstyrrede biologiske membraner.

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Neuroscience biofysik ionkanaler single-kanal optagelse NMDA-receptorer gating elektrofysiologi patch-clamp kinetisk analyse
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