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Identifikation af Protein Interaction Partnere i pattedvrceller Brug SILAC-immunpræcipitations Kvantitative Proteomics
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Identifikation af Protein Interaction Partnere i pattedvrceller Brug SILAC-immunpræcipitations Kvantitative Proteomics

Article DOI: 10.3791/51656-v • 12:53 min • July 6th, 2014
July 6th, 2014 •
1, 1
1Division of Virology, Department of Pathology, University of Cambridge

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SILAC immunpræcipitationseksperimenter repræsenterer et stærkt middel til at opdage nye protein: protein interaktioner. Ved at tillade nøjagtig relative kvantificering af protein overflod i både kontrol og prøver, sande interaktioner let kan skelnes fra eksperimentelle forureninger, og lav affinitetsvekselvirkning bevaret gennem brug af mindre strenge buffer forhold.

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Biokemi massespektrometri vævsdyrkningsteknikker isotop mærkning SILAC Stald isotopmærkning af aminosyrer i cellekultur proteomik Interactomics immunoudfældning pulldown eIF4A GFP nanotrap orbitrap
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