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Estudar DNA Looping por Single-Molecule FRET
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Estudar DNA Looping por Single-Molecule FRET

Article DOI: 10.3791/51667-v • 11:27 min • June 28th, 2014
June 28th, 2014 •
1, 1
1School of Physics, Georgia Institute of Technology

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Este estudo apresenta um procedimento experimental detalhado para medir a dinâmica de loop de DNA de cadeia dupla usando uma única molécula de fluorescência Resonance Energy Transfer (FRET). O protocolo também descreve como extrair a densidade de probabilidade looping chamado o factor de J.

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Biologia Molecular looping DNA fator J molécula única FRET Gel mudança mobilidade a curvatura do ADN cadeia sem-fim-como
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