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Journal / Immunology and Infection / In Situ Detectie van autoreactieve CD4 T-cellen…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ Detectie van autoreactieve CD4 T-cellen in de hersenen en het hart met behulp van Hoofdhistocompatibiliteitscomplex Klasse II Dextramers
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In Situ Detectie van autoreactieve CD4 T-cellen in de hersenen en het hart met behulp van Hoofdhistocompatibiliteitscomplex Klasse II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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Is beschreven het protocol tot zelf-reactieve CD4 T-cellen te detecteren in de hersenen en het hart door directe kleuring met hoofdhistocompatibiliteitscomplex klasse II dextramers in dit rapport. Voor uitgebreide analyse is een betrouwbare methode om de frequentie van antigeen-specifieke CD4 + T-cellen in situ sommen ook ontwikkeld.

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Immunologie dextramers; MHC klasse II;
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