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Helcelle-patch-clamp Optagelser fra Morphologically- og Neurochemically identificerede Hippocampale interneuroner
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Helcelle-patch-clamp Optagelser fra Morphologically- og Neurochemically identificerede Hippocampale interneuroner

Article DOI: 10.3791/51706-v • 14:37 min • September 30th, 2014
September 30th, 2014 •
1, 1, 1
1Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin

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Corticale netværk styres af en lille, men varieret sæt hæmmende interneuroner. Funktionel undersøgelse af interneuroner kræver derfor målrettet optagelse og stringent identifikation. Beskrevet her er en kombineret tilgang, der involverer hele-celle optagelser fra en enkelt eller synaptisk koblede par af neuroner med intracellulær mærkning, post-hoc morfologiske og immuncytokemisk analyse.

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Neuroscience elektrofysiologi akut skive hel-celle patch-clamp optagelse neuronal morfologi immunhistokemi parvalbumin hippocampus hæmning GABAerge interneuroner synaptisk transmission IPSC GABA-B-receptor
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