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Um Protocolo de vídeo de infecção retroviral na Primária Intestinal organóide Cultura
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Um Protocolo de vídeo de infecção retroviral na Primária Intestinal organóide Cultura

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Este protocolo explica cultura organóide Lgr5-positve primário eo desempenho subseqüente de transdução retroviral. Isto permite que a sobre-expressão de Cre-indutível ou knockdown do transgene entregue e permite estudos funcionais a serem realizadas no novo em organotípica vitro sistema modelo.

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Genetics Edição 90 Retrovírus Lentivírus cultura organóide Lgr5 Intestino 3Rs
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