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Journal / Immunology and Infection / Imagens em tempo real de endoteliais junções…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Imagens em tempo real de endoteliais junções célula-célula durante Neutrophil Transmigração Sob Fisiológica Fluxo
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Imagens em tempo real de endoteliais junções célula-célula durante Neutrophil Transmigração Sob Fisiológica Fluxo

Article DOI: 10.3791/51766-v • 11:26 min • August 14th, 2014
August 14th, 2014 •
*1, *1, 1, 1
1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam
* These authors contributed equally

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Os leucócitos atravessam a monocamada endotelial utilizando o paracelular ou o percurso intracelular. Foi desenvolvido um ensaio simples para seguir a distribuição de juncional endógeno VE-caderina e PECAM-1 durante a migração transendotelial de leucócitos sob fluxo fisiológico para discriminar entre as duas vias de transmigração.

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Imunologia leucócitos Cordão Veia células endoteliais (HUVEC) transmigração VE-caderina PECAM-1 endotélio transcelular paracellular
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