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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering, identifikation og oprensning af murine thymusepitelmonolag Celler
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
Journal (Immunology and Infection)
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses
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Isolering, identifikation og oprensning af murine thymusepitelmonolag Celler

Article DOI: 10.3791/51780-v • 07:20 min • August 8th, 2014
August 8th, 2014 •
1, 1
1Department of Laboratory Medicine & Pathology, Center for Immunology, University of Minnesota

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Her beskriver vi en effektiv fremgangsmåde til isolering, identifikation og oprensning af muse thymiske epitelceller (TECs). Protokollen kan bruges til undersøgelser af thymus funktion for en normal T-celle udvikling, thymus-dysfunktion, og T-celle rekonstitution.

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