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A Biochip eletroquímica microfluídica à base de Análise de hibridação DNA livre-Label

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Nós apresentamos um biochip electroquímica à base de microfluidos para a detecção de hibridação de ADN. Após a funcionalização ADNcs sonda, a especificidade, a sensibilidade, e o limite de detecção são estudadas com alvos de ADNcs complementares e não complementares. Os resultados ilustram a influência dos eventos de hibridação de ADN no sistema electroquimico, com um limite de detecção de 3,8 nM.

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Bioengenharia espectroscopia de impedância eletroquímica hibridização de DNA biosensor biochip microfluídica detecção de livre-label difusão restrita microfabricação
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