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JoVE Journal
Biology

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小鼠睾丸活体显微注射和电穿孔
 
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小鼠睾丸活体显微注射和电穿孔

Article DOI: 10.3791/51802-v 08:39 min August 23rd, 2014
August 23rd, 2014

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Summary

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Click here for the English version.

本文介绍了显微注射和小鼠睾丸中电在体内的睾丸小鼠细胞转染技术,研究精子的独特工艺。所提出的方案涉及以下步骤:玻璃毛细管制备,经由输出管显微注射,并通过电穿孔转染。

Tags

分子生物学,第90,电,染,显微注射,睾丸,精子,精子,再现
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