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Journal / Immunology and Infection / Hurtig analyse af kromosomafvigelser i Mouse B…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Hurtig analyse af kromosomafvigelser i Mouse B lymfocytter ved PNA-FISH
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Hurtig analyse af kromosomafvigelser i Mouse B lymfocytter ved PNA-FISH

Article DOI: 10.3791/51806-v • 07:54 min • August 19th, 2014
August 19th, 2014 •
1, 1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

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DNA-reparation veje er afgørende for opretholdelse af genomisk integritet og forhindre mutation og kræft. Målet med denne protokol er at kvantificere genomisk ustabilitet ved direkte observation af kromosomafvigelser i metafasespredninger fra muse-B-celler ved hjælp af fluorescerende in situ-hybridisering (FISH) til telomeriske DNA gentagelser.

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Immunologi genomisk ustabilitet DNA-reparation mus metafasespredning FISK primær kultur
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